Stroke, a cerebrovascular disease (CVD), is the main cause of temporary or permanent neurologic disabilities in individuals aged 50 years and older. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper.įunding: This research was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (BR), CNPq-465259/2014-6, and CNPq-400856/2016-6 to LFG São Paulo State Research Support Foundation, FAPESP: 2014/50983-3, and FAPESP:2016/21470-3 to LFG. Received: OctoAccepted: JPublished: July 5, 2018Ĭopyright: © 2018 da Silva et al. PLoS ONE 13(7):Įditor: Johannes Boltze, Fraunhofer Research Institution of Marine Biotechnology, GERMANY (2018) Evaluation of temperature induction in focal ischemic thermocoagulation model. We conclude that a TCI temperature of 400☌ causes permanent ischemic stroke and motor deficit.Ĭitation: da Silva H, Nucci MP, Mamani JB, Mendez-Otero R, Nucci LP, Tannus A, et al. The MRI and TTC analyses revealed that ischemic brain lesions expressively evolved, especially at TCI temperatures of 300☌ and 400☌, and significant motor deficits were observed as the animals showed a decrease frequency of movement and an asymmetric pattern. In the second experiment, five groups ( n = 5 each) were analyzed by MRI (basal and 24 h after TCI) and behavioral tests (basal and seven days after TCI) with the control group added for the surgical effects. In the first experiment, the animals were divided into four groups ( n = 5 each): one sham surgery group and three ischemic lesion groups having thermocoagulation induction (TCI) temperatures of 200☌, 300☌, and 400☌, respectively, with blood perfusion (basal and 30 min after TCI) and 2,3,5-Triphenyl-tetrazolium chloride (TTC) evaluation at 2 h after TCI. We used three-month-old male Wistar rats, weighing 300–350 g. We investigated parameters such as temperature and thermal dissipation in the brain tissue during induction and analyzed real-time blood perfusion, histological changes, magnetic resonance imaging (MRI), and motor behavior in a permanent ischemic stroke model. This study analyzed the best conditions for inducing a focal ischemic lesion by thermocoagulation. SEDACOM will automatically recognize the number of systems connected.Ĭonfigure & control your device (IR actimeter, Treadmill, etc.The thermocoagulation model, which consists of focal cerebral ischemia with craniectomy, is helpful in studying permanent ischemic brain lesions and has good reproducibility and low mortality. An additional hub or board with serial ports (not included) can be installed to expand the number of connections. SEDACOM can adquire data simultaneously from up to 9 devices, limited only by the number of series ports available on the computer. Optional accessories allow use of an USB stick to conduct experiments with a laptop. The name of SEDACOM comes from SErial DAta COMmunication, due to the direct communication via an RS232 serial port connection between Panlab devices and computers. SEDACOM can be used with a wide range of Panlab products for measuring physiology and behaviour in small laboratory animals (motor activity, pain sensitivity, body temperature, memory etc.) SEDACOM increases the functionalities of the devices, saving and listing automatically all the data of the current or stored sessions. The SEDACOM 2.0 is a very easy, convenient and cost-saving data transfer software providing an ideal environment for visualizing the registered data on a computer and exporting them in a format that simplifies any further post-analysis processes.
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